BNCC
Escherichia coli DH5α(pET28a-EGFP)
SKU 359701
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli DH5α(pET28a-EGFP) |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | ① Thaw Frozen vials in a water bath (37°C), shaking vigorously to dissolve; ② Disinfect the outer surface of the vial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire thawed solution, and Agar plate onto 1-2 agar plates (approx. 200μL per Agar plate); ④ Spread evenly and incubate under the specified conditions. |
| Culture Conditions | 37°C; 18-24 hours; aerobic |
| Provided Formats | Frozen vial |
| Culture Medium No. | 361023 |
| Culture Medium Name | LB Medium with 50μg/mL Kanamycin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | -80°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.