BNCC
Escherichia coli DH5α(pET-52b)
SKU 396511
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli DH5α(pET-52b) |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | ① Prepare 1 vial of sterile water and 2 90-mm resistance plates; ② Disinfect the surface of the ampoule, open it in a safety cabinet, scorch the top with a酒精灯, then quickly add a drop of sterile water to break it open, and subsequently crush it with tweezers; ③ Pipette 0.5 mL of sterile water into the lyophilized vial; after complete dissolution, streak the solution onto the resistance plates at 200 μL per Agar plate; ④ Incubate the resistance plates under the specified conditions for 18–24 h before observation (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB resistance medium; incubate overnight on a shaking incubator at 37°C; ⑥ Depending on experimental requirements, pipette an appropriate volume of the bacterial culture for plasmid extraction |
| Culture Conditions | 37°C; 18–24 hours; aerobic |
| Provided Formats | Frozen vial |
| Culture Medium No. | 362905 |
| Culture Medium Name | LB Medium with 100μg/mL Ampicillin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | –80°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.