BNCC
Escherichia coli DH5α(pET-24a)
SKU 396949
Bioware
A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli DH5α(pET-24a) |
|---|---|
| Material Type | Recombinant Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | ① Prepare 1 sterile water tube and 2 90mm resistant plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Draw 0.5mL of sterile water and transfer it into a freeze-drying tube. After sufficient dissolution, apply the solution onto resistant plates at a rate of 200 μL per Agar plate; ④ Place the resistant Agar plate under the specified conditions for cultivation for 18-24 hours and observe it (sealing and wrapping the Agar plate is prohibited); ⑤ Select a single colony and inoculate it into 50mL of liquid LB resistant medium. Incubate overnight at 37 °C on a shaker; ⑥ According to experimental requirements, extract an appropriate amount of bacterial solution for plasmid extraction |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | Frozen vial |
| Culture Medium No. | 361023 |
| Culture Medium Name | LB Medium with 50μg/mL Kanamycin |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C) |
| Application | Molecular biology research, recombinant DNA studies, method development, and laboratory research. |
|---|---|
| Storage Conditions | -80 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.