Escherichia coli DH5α(pBiT1.1-N [TK-LgBiT]) — BNCC 359211

BNCC

Escherichia coli DH5α(pBiT1.1-N [TK-LgBiT])

SKU 359211

Bioware

A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.

Technical Specifications

OrganismEscherichia coli DH5α(pBiT1.1-N [TK-LgBiT])
Material TypeRecombinant Microbial Strain
Preservation FormFreeze dried pellet
Culture Method① Prepare 1 vial of sterile water and 2 90mm resistant plates; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture; break ampoule with forceps; ③ Pipette 0.5 mL sterile water into lyophilized tube, dissolve completely, then spread solution onto antibiotic plates (200 μL/Agar plate); ④ Incubate the resistance plates under specified conditions for 18-24 hours before observation (do not seal or wrap plates); ⑤ Pick a single colony and inoculate into 50 mL LB resistance liquid medium; incubate overnight at 37°C on a shaking incubator; ⑥ Extract plasmids from an appropriate volume of the bacterial culture as required by the experiment
Culture Conditions37°C; 18-24h; aerobic
Provided FormatsFreeze dried pellet
Culture Medium No.361029
Culture Medium NameLB Medium with 50μg/mL Ampicillin
Culture Medium CompositionYeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25°C)
ApplicationMolecular biology research, recombinant DNA studies, method development, and laboratory research.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.