BNCC
Escherichia coli BL21(DE3)pLysS
SKU 353807
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Escherichia coli BL21(DE3)pLysS |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, yellow on the front, light color, raised in the middle, smooth surface, moist texture, easy to pick |
| Culture Method | ① Prepare 1-2 of the above plates; ② Open the safety cabinet and follow the arrow indication on the cap of the penicillin bottle to open the strain and reconstitution solution; ③ Add 1mL of the complex solution to the Freeze dried pellet, cover with a rubber stopper, and shake to dissolve; ④ Take 200 μL of bacterial suspension separately and inject it into two plates, apply evenly; ⑤ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Culture Conditions | 37 °C; 18-24h; aerobic |
| Provided Formats | Freeze dried pellet; Agar Slant; Bacterial suspension; Agar plate |
| Culture Medium No. | 360993 |
| Culture Medium Name | LB Medium with 50μg/mL Chloramphenicol |
| Culture Medium Composition | Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, Agar:15.0g, Chloramphenicol:50μg/mL, pH:7.0(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | -20 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.