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BNCC

Enterococcus hirae

SKU 137527

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismEnterococcus hirae
Material TypeMicrobial Strain
Preservation Formfreeze-dried powder
Morphological CharacteristicsSize: 0.5-1mm Shape: Circular Margin: Entire Transparency: Opaque Color: Grayish white Elevation: Raised center Surface: Bright and smooth Texture: Moist Gram stain: Gram-positive (blue-purple), rod-shaped
Culture Method① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Culture Conditions37 °C; 24-48 hours; anaerobic
Provided FormatsFreeze dried powder; Bacterial suspension; Plate culture
Culture Medium No.352238
Culture Medium NameMRS agar medium
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.