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Enterococcus faecalis — BNCC 194769

BNCC

Enterococcus faecalis

SKU 194769

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismEnterococcus faecalis
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColony diameter: 0.5–1 mm; circular, with neat edges; opaque, grayish-white on top; centrally raised; smooth surface; easily liftable; Gram-positive (blue-violet); cocci
Culture Method① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5 mL of liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2 mL of bacterial suspension onto a Agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once growth appears.
Culture Conditions30°C; 18-24h; aerobic
Provided FormatsBacterial suspension; Agar plate
Culture Medium No.352238
Culture Medium NameMRS agar medium
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, C6H8O7.(NH4)2:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.