BNCC
Dengue virus type 3
SKU 390326
Bioware
A characterized viral material intended for controlled virological and molecular studies.
Suitable for assay development, detection-method verification, comparative testing, and laboratory research under appropriate biosafety conditions.
Technical Specifications
| Organism | Dengue virus type 3 |
|---|---|
| Material Type | Virus Material |
| Preservation Form | Cryovial |
| Culture Method | ① Inoculation procedure: Seed host cells into T25 flasks 24–48 hours prior to inoculation, and prepare for viral inoculation when the host cell density reaches 80–90%; Remove the cell culture medium and inoculate 100–500 μL of viral suspension. Allow adsorption for 1–2 hours under culture conditions, gently shaking the T25 flask every 15–30 minutes to ensure even viral distribution; add 6 mL of viral growth medium to complete adsorption, transfer to the incubator for continued culture, and monitor cytopathic effects periodically; ② Virus Harvesting: Once all host cells exhibit cytopathic effects, begin harvesting the viral supernatant. During harvesting, freeze-thaw the cells 1–2 times, then filter through a 0.22 μm pore size filter; the collected filtrate constitutes the viral supernatant. |
| Culture Conditions | 37°C; 5% CO₂ + 95% air |
| Provided Formats | Cryovial |
| Culture Medium No. | 361988 |
| Culture Medium Name | EMEM Complete Medium (with 2% FBS) |
| Culture Medium Composition | 98% EMEM+2% FBS |
| Application | Virology research, assay development, method verification, and laboratory quality control. |
|---|---|
| Storage Conditions | Liquid nitrogen |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.