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BNCC

Corynebacterium diphtheriae (Kruse) Lehmann and Neumann

SKU 316506

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismCorynebacterium diphtheriae (Kruse) Lehmann and Neumann
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsThe colonies are 0.5–1 mm in diameter, circular with entire margins, and opaque. The colony surface is grayish-white, convex, smooth, shiny, and moist in texture. They are easily picked up. The cells are Gram‑positive (blue‑violet) and rod‑shaped (bacilli). The culture is pure.
Culture Method① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μL per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Culture Conditions37 °C; 18-24h; 5% CO2
Provided FormatsFreeze dried pellet; Agar plate
Culture Medium No.352241
Culture Medium NameColumbia blood agar plate
Culture Medium CompositionSpecial Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50°C), pH:7.3±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.