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BNCC

Clostridium sporogenes

SKU 104015

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismClostridium sporogenes
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsThe colony has a diameter of 0.5–1 mm, appearing circular with neat edges and an opaque texture. It is grayish-white on the obverse side, with a raised center, smooth surface, and moist consistency. The colony is easily picked up, tests Gram-positive (showing blue-violet staining), and consists of rod-shaped bacteria.
Culture Method① Prepare two of the above-mentioned agar plates and sterile water (pre-equilibrated in an Anaerobic environment for 24 hours);② Disinfect the surface of the ampoule tube, open it in a biosafety cabinet, flame the top with an alcohol lamp, then quickly drip sterile water onto it to crack it open, and use tweezers to break it apart;③ Aspirate 0.5 mL of sterile water, inject it into the freeze-dried tube, fully dissolve the bacterial powder, then transfer it onto two agar plates, 200 μL per plate, and spread evenly;④ Place the plates under the aforementioned culture conditions for incubation. The bacterial strain can be used once growth is observed.
Culture Conditions37 °C; 18-24h; Anaerobic
Provided FormatsFreeze dried pellet; Bacterial suspension; Plate culture
Culture Medium No.352241
Culture Medium NameColumbia blood agar plate
Culture Medium CompositionSpecial Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50°C), pH:7.3±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.