BNCC
Clostridium sporogenes (Metchnikoff) Bergey et al.
SKU 266047
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Clostridium sporogenes (Metchnikoff) Bergey et al. |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colonies measure 1-2 mm in diameter, are irregular in shape with filamentous margins, and are opaque. They exhibit a grayish-white pigmentation on the surface, have a flat morphology, and are rough and dull in appearance. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli). Purity: pure. |
| Culture Method | ① Prepare 1-2 fresh agar plates as described above (pre-conditioned in an anaerobic environment for 24 h to remove oxygen).② In a biosafety cabinet, open the vial and briefly flame the top with an alcohol burner, then immediately add sterile water to break the seal. Use sterile forceps to crush the vial.③ Aspirate 0.5 mL of liquid medium or sterile water (pre-conditioned in an anaerobic environment for 24 h to remove oxygen) into the freeze‑dried vial, thoroughly dissolve the contents, and mix well.④ Transfer 0.2 mL of the resulting bacterial suspension onto an agar plate, spread evenly, and repeat to inoculate a second Agar plate.⑤ Incubate the plates under the specified culture conditions. The strain is ready for use once growth appears. |
| Culture Conditions | 37 °C; 18-24h; anaerobic |
| Provided Formats | Freeze dried pellet; Bacterial suspension; Agar plate |
| Culture Medium No. | 352241 |
| Culture Medium Name | Columbia blood agar plate |
| Culture Medium Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50°C), pH:7.3±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.