BNCC
Clostridium perfringens
SKU 387045
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Clostridium perfringens |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | Colony diameter: 1-2 mm; circular, irregular margins; opaque, grayish-white surface; flat morphology; easily liftable; Gram-positive (blue-violet); bacilli |
| Culture Method | ① Prepare two plates as above (equilibrated in anaerobic conditions for 24h); ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture; break open with forceps; ③ Pipette approximately 0.5 mL sterile water (pre-equilibrated anaerobically for 24h) into the lyophilized vial. Thoroughly dissolve the bacterial powder, then inoculate 2 plates with 200μL/Agar plate each, spreading evenly; ④ Incubate plates under the aforementioned conditions. |
| Culture Conditions | 37°C; 18-24h; anaerobic |
| Provided Formats | Bacterial suspension; Agar plate |
| Culture Medium No. | 352241 |
| Culture Medium Name | Columbia blood agar plate |
| Culture Medium Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50°C), pH:7.3±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.