Clostridioides difficile (Prevot) Lawson et al. — BNCC 378701

BNCC

Clostridioides difficile (Prevot) Lawson et al.

SKU 378701

Bioware

A genetically modified microbial culture carrying defined recombinant characteristics for controlled experimental use.
Suitable for molecular biology workflows, expression studies, method verification, and laboratory quality control.

Technical Specifications

OrganismClostridioides difficile (Prevot) Lawson et al.
Material TypeRecombinant Microbial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsThe colony has a diameter of 1-2mm, irregular shape, irregular edges, opaque, white front, raised middle, smooth surface, moist texture, G+(blue purple), Bacillus, purity: pure
Culture Method①Prepare 1-2 fresh agar plates (placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After fully dissolving, transfer it back to the liquid test tube and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Culture Conditions37 °C; 24-48 hours; anaerobic
Provided FormatsFreeze dried pellet; Agar plate
Culture Medium No.359963
Culture Medium NameTrypticase Soy Blood Agar
Culture Medium CompositionTryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Sterile Defibrinated Sheep Blood:5%, Agar:15.0g, pH:7.3±0.2(25°C)
ApplicationMolecular biology research, recombinant DNA studies, method development, and laboratory research.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.