BNCC
Chlamydia trachomatis Serovar J
SKU 377508
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Chlamydia trachomatis Serovar J |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | ①Inoculation procedure: 24-48 hours before inoculation, transfer the frozen host cells into T25 flask, remove the cell culture medium when the host cell density reaches 80-90%, and inoculate 100-500 μL of virus solution. Adsorb under growth conditions for 1-2 hours, gently shake the T25 flask every 15-30 minutes to make the virus distribution more uniform; Add 6mL of virus growth medium to end adsorption, transfer to the incubator for further cultivation, and regularly observe the cytopathy; ② Virus collection: Once all host cells have developed lesions, virus liquid collection can begin. When collecting, the cells are repeatedly frozen and thawed 1-2 times, and then filtered through a 0.22 μ m pore size filter. The collected filtrate is the virus solution. |
| Culture Conditions | 37°C; 5% CO₂ + 95% air |
| Provided Formats | Frozen vial |
| Culture Medium No. | 361988 |
| Culture Medium Name | EMEM Complete Medium (with 2% FBS) |
| Culture Medium Composition | 98% EMEM+2% FBS |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | Liquid nitrogen |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.