BNCC
CD3+(Positive) (only available for cryopreservation)
SKU 384584
Bioware
A characterized cultured-cell model intended for controlled in vitro research and analytical applications.
Suitable for cell-based assay development, method verification, comparative studies, and laboratory research.
Technical Specifications
| Organism | CD3+(Positive) |
|---|---|
| Material Type | Cell Line |
| Preservation Form | frozen vial |
| Morphological Characteristics | Lymphocyte like, single cell |
| Culture Method | Resuscitation steps: ① Remove the cryotube from liquid nitrogen or -80 °C refrigerator and place it in PE gloves, quickly immerse it in a 37 °C water bath, shake the cryotube to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete culture medium on a super clean bench, centrifuge at 1000-1200rpm for 3-5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete culture medium. ③ Then add the cell suspension to a T25 bottle containing 6-7mL of complete culture medium and culture it in an incubator. It is recommended to cultivate the bottle vertically. |
| Culture Conditions | 37 °C; 5% CO2+95% air |
| Provided Formats | cryovial |
| Culture Medium No. | 338360 |
| Culture Medium Name | RPMI-1640 Complete Medium (with 10% FBS) |
| Culture Medium Composition | 90%RPMI-1640+10%FBS |
| Application | Cell culture research, method development, assay validation, and laboratory research. |
|---|---|
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.