BNCC
Borrelia burgdorferi B31
SKU 370576
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Borrelia burgdorferi B31 |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | ① Dissolve the Frozen vial in a 37 °C water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into a test tube containing 5-6mL of culture medium; ④ Then transfer to the above cultivation conditions for cultivation. ⑤ After 10 days of cultivation, the broth culture is not turbid, and there will be a slight precipitate at the bottom after centrifugation (Borrelia burgdorferi is a fragile and sensitive microorganism that requires the use of suitable culture medium for growth. Rabbit serum is crucial for the growth of this organism. The use of freshly prepared culture medium can promote growth. Culture media that have been prepared for more than a month should not be used.) |
| Culture Conditions | 37 °C; 7-10 days; 3% -5% O2, 10% CO2 |
| Provided Formats | Frozen vial |
| Culture Medium No. | 390982 |
| Culture Medium Name | ATCC Medium 1914 |
| Culture Medium Composition | HEPES(Sigma H-3375):5.64g, Neopeptone:4.7g, Na3C6H5O7:0.7g, Glucose:5.64g, NaHCO3:2.0g, TC-Yeast Extract(BD 255772):2.0g, C3H3NaO3:0.75g, N-Acetylglucosamine:0.37g, Bovine Serum Albumin, Fraction V:47.0g, CMRL 1066 10X(without glutamine and sodium bicarbonate):100.0ml, Rabbit Serum(heat-inactivated):60.0ml, Deionized Water:840ml |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | -80 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.