Skip to content

BNCC

Bifidobacterium longum

SKU 341605

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismBifidobacterium longum
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsThe colonies are 1-2 mm in diameter, circular with entire margins, and opaque. The colony surface is cream-white, convex, smooth, shiny, and moist in texture. They are easily picked up. The cells are Gram-positive (blue-violet) and rod-shaped (bacilli). The culture is pure.
Culture Method① Prepare two of the aforementioned agar plates (pre‑equilibrated under anaerobic conditions for 24 hours).② After surface disinfection, open the ampoule in a biosafety cabinet. Flame the top of the ampoule with an alcohol burner, then quickly apply a drop of sterile water to crack it, and gently break the ampoule using sterile forceps.③ Aspirate 0.5 mL of sterile water (pre‑equilibrated under anaerobic conditions for 24 hours) and inject it into the lyophilized vial. Thoroughly resuspend the bacterial pellet, then inoculate 200 µL onto each of the two plates and spread evenly.④ Incubate the plates under the specified culture conditions. The culture is ready for use once growth is observed.
Culture Conditions37 °C; 48-72 hours; anaerobic
Provided FormatsFreeze dried pellet; Bacterial suspension; Agar plate
Culture Medium No.352254
Culture Medium NameBBL agar medium
Culture Medium CompositionPeptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.