BNCC
Bifidobacterium breve
SKU 185357
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Bifidobacterium breve |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colonies are 1-2 mm in diameter, circular with irregular margins, and opaque. The obverse side is creamy white, convex (raised at the center), with a smooth and moist texture. The cells are Gram-positive (staining blue-violet), rod-shaped. Purity: Pure. |
| Culture Method | ① Prepare 1-2 fresh agar plates as specified above (pre-reduced in an anaerobic environment for 24 hours).② Within a biological safety cabinet, surface-sterilize and open the glass ampoule. Briefly flame the neck, then immediately apply a drop of sterile water to induce thermal cracking. Carefully open it using sterile forceps.③ Aspirate 0.5 mL of sterile, pre-deoxygenated water (similarly pre-reduced for 24 hours) and inject it into the freeze-dried vial. Mix thoroughly for complete resuspension. Transfer the entire suspension into a tube containing liquid medium and mix well.④ Inoculate 0.2 mL of the resulting bacterial suspension onto each pre-reduced agar plate and spread evenly. Prepare a duplicate Agar plate following the same method.⑤ Incubate all plates under the aforementioned anaerobic conditions. The culture is ready for use when visible growth appears. |
| Culture Conditions | 37 °C, 48-72 hours, anaerobic |
| Provided Formats | Freeze dried pellet; Bacterial suspension; Agar plate |
| Culture Medium No. | 360803 |
| Culture Medium Name | Enhanced Clostridium agar medium |
| Culture Medium Composition | Yeast Extract:3.0g, Beef Extract Powder:10.0g, Glucose:5.0g, Pancreatic Casein Peptone:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C3H7NO2S.HCl):0.5g, CH3COONa:3.0g, Agar:12.5g, pH:6.8±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.