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BNCC

Bifidobacterium bifidum

SKU 186522

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismBifidobacterium bifidum
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColony diameter: 0.5–1 mm; circular with irregular margins; upper surface grayish-white, centrally convex; surface dull gray, dry texture; Gram-positive (blue-violet), bacilli; purity: pure
Culture Method① Prepare 1 tube containing 5-10mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL liquid medium into the freeze-dried vial, dissolve completely, then return to the liquid test tube and mix thoroughly; ④ Pipette 0.2mL bacterial suspension onto plates, spread evenly; repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the strain grows.
Culture Conditions37°C; 3-4 days; anaerobic
Provided FormatsBacterial suspension; Agar plate
Culture Medium No.352254
Culture Medium NameBBL agar medium
Culture Medium CompositionPeptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.