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Bifidobacterium bifidum — BNCC 367988

BNCC

Bifidobacterium bifidum

SKU 367988

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismBifidobacterium bifidum
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsThe colonies measure 0.5-1 mm in diameter, are circular with regular margins, and are opaque. They exhibit a creamy-white pigmentation on the surface, have a raised center, and are smooth and firm in texture. No pigment is produced. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli). Purity: pure.
Culture Method① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μL per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Culture Conditions37 °C; 48-72 hours; anaerobic
Provided FormatsFreeze dried pellet; Bacterial suspension; Agar plate
Culture Medium No.360833
Culture Medium NameBBL Fluid Medium
Culture Medium CompositionPeptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0g, pH:7.0±0.1(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.