BNCC
Bifidobacterium bifidum
SKU 336858
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Bifidobacterium bifidum |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colony diameter is 1-2mm, circular, with neat edges, milky white on the front, raised in the middle, smooth surface, bright surface, moist texture, no pigment, G+(blue purple), Bacillus, purity: pure |
| Culture Method | ① Prepare two of the abovementioned agar plates (pre-placed in an anaerobic environment for 24 hours for oxygen removal).② Surface-sterilize the ampoule vial, then open it inside a biological safety cabinet. Flame the top of the vial with an alcohol burner, and immediately apply a drop of sterile water to crack it. Then, use forceps to carefully break it open.③ Aspirate 0.5 mL of sterile water (pre-placed in an anaerobic environment for 24 hours for oxygen removal) and inject it into the lyophilized vial. Thoroughly resuspend the bacterial powder. Then, transfer the suspension onto the two prepared plates, 200 μL per Agar plate, and spread evenly.④ Incubate the plates under the specified culture conditions mentioned above. The bacterial strain is ready for use once growth is observed. |
| Culture Conditions | 37 °C; 3-4 days; anaerobic |
| Provided Formats | Freeze dried pellet; Bacterial suspension |
| Culture Medium No. | 352254 |
| Culture Medium Name | BBL agar medium |
| Culture Medium Composition | Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.