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BCG-pMV361-eGFP — BNCC 391132

BNCC

BCG-pMV361-eGFP

SKU 391132

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismBCG-pMV361-eGFP
Material TypeMicrobial Strain
Preservation FormFrozen vial
Morphological CharacteristicsGreen fluorescence
Culture Method① Dissolve the Frozen vials in a 37 °C water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μL per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. During the cultivation process, the agar plate needs to be kept moist, and 7H10 broth can be added.
Culture Conditions37 °C; 2-3 weeks; aerobic
Provided FormatsBacterial suspension; Agar plate; Frozen vial tube
Culture Medium No.393078
Culture Medium NameMiddlebrook 7H9 Agar Plate with 25μg/mL Kanamycin (Ready-to-use)
Culture Medium Composition(NH4)2SO4:0.5g, KH2PO4:1.0g, Na2HPO4:2.5g, Na3C6H5O7:0.1g, MgSO4:0.05g, CaCl2:0.0005g, ZnSO4:0.001g, CuSO4:0.001g, L-Glutamic Acid(C5H9NO4):0.5g, C6H8FeNO7:0.04g, Vitamin B6(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.0005g, ADC Supplement:10%(added at 50°C), Agar:18.0g, pH:6.6±0.2(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions-80 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.