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Bacillus thuringiensis — BNCC 133175

BNCC

Bacillus thuringiensis

SKU 133175

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismBacillus thuringiensis
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsSize: 1–2 mm Shape: Circular Margins: Irregular Transparency: Opaque Color: Grayish-white Elevation: Flat Surface: Dull and rough Texture: Dry
Culture Method① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, flame the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2 mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows.
Culture Conditions30°C, 18-24h, aerobic
Provided FormatsAgar Slant; Bacterial suspension; Agar plate
Culture Medium No.352239
Culture Medium NameNutrient Agar
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.