BNCC
Bacillus subtilis subsp. subtilis
SKU 133116
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Bacillus subtilis subsp. subtilis |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | Colony diameter: 0.5–1mm; circular, with neat edges; opaque, grayish-white on the upper surface; flat morphology; smooth surface; easily liftable; G+ (blue-violet); bacillus |
| Culture Method | ① Prepare 1 test tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, flame the top with an alcohol lamp, then quickly add sterile water to break it; subsequently crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid test tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows. |
| Culture Conditions | 30°C, 18-24h, aerobic. |
| Provided Formats | Agar Slant; Bacterial suspension; Agar plate |
| Culture Medium No. | 360795 |
| Culture Medium Name | Nutrient Blood Agar |
| Culture Medium Composition | Beef Extract:3.0g, Peptone:10.0g, NaCl:5.0g, Agar:15.0g, pH:7.0(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.