Skip to content

BNCC

Bacillus subtilis subsp. Spizizenii

SKU 186162

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismBacillus subtilis subsp. Spizizenii
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsThe colony diameter is 1-2mm, circular, with neat edges, opaque, gray white front, flat shape, rough surface, gray surface, dry texture, difficult to pick/cut, G+(blue purple), Bacillus subtilis
Culture Method① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into the freeze-drying tube, dissolve thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Culture Conditions30 °C; 18-24h; aerobic
Provided FormatsFreeze dried pellet; Agar Slant; Bacterial suspension; Agar plate
Culture Medium No.352239
Culture Medium NameNutrient Agar
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8 °C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.