BNCC
Bacillus subtilis spore counting standard substance
SKU 359088
Bioware
A standardized microbiological quality-control material designed to provide a defined analytical challenge.
Suitable for method verification, recovery studies, detection or enumeration performance assessment, and routine quality control.
Technical Specifications
| Organism | Reference material of bacillus subtilis spores count |
|---|---|
| Material Type | Microbiological Quality Control |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | The colony diameter is 2-4mm, circular, with wavy edges, opaque, gray white on the front, flat in shape, rough on the surface, dry in texture, G+(blue purple), Bacillus |
| Culture Method | ① Cultivate the bacteria into a bacterial suspension according to the cultivation conditions first; ② Measure the absorbance of bacterial suspension and calculate the initial concentration of bacterial suspension according to the standard formula; ③ The actual concentration after freeze-drying is obtained by multiplying the initial concentration by the survival rate; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then divide and freeze dry it |
| Culture Conditions | 37 °C, aerobic, 18-24h |
| Provided Formats | Penicillin bottle |
| Culture Medium No. | 352239 |
| Culture Medium Name | Nutrient Agar |
| Culture Medium Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C) |
| Pack Configuration | Single Vial |
|---|
| Application | Microbiological method verification, enumeration, and laboratory quality control. |
|---|---|
| Storage Conditions | -20 °C, dark storage |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.