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BNCC

Bacillus subtilis

SKU 188080

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismBacillus subtilis
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColony diameter: 1-2 mm; circular with irregular margins; opaque, grayish-white upper surface; flattened morphology; dull, rough surface; moist texture; easily lifted; Gram-positive (blue-violet); bacilli
Culture Method① Prepare 1-2 plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then rapidly add sterile water to rupture. Crush with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube. After complete dissolution, dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Culture Conditions30°C; 18-24h; aerobic
Provided FormatsAgar Slant; Bacterial suspension; Agar plate
Culture Medium No.352239
Culture Medium NameNutrient Agar
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.