BNCC
Bacillus subtilis
SKU 188023
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | Bacillus subtilis |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Freeze dried pellet |
| Morphological Characteristics | Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: Grayish-white Elevation: Flat Surface: Rough Texture: Dry |
| Culture Method | ① Prepare 1 tube containing 5–10 mL liquid medium and 2 plates; ② Open in a safety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it, and crush it with forceps; ③ Pipette 0.5 mL liquid medium into the freeze-dried vial, dissolve thoroughly, then return to the liquid tube and mix well; ④ Pipette 0.2mL of the bacterial suspension onto a plate and spread evenly. Repeat twice to obtain two plates. ⑤ Incubate all liquid tubes and plates under the specified conditions. The culture is ready for use once the bacterial strain grows. |
| Culture Conditions | 30°C; 18-24h; Aerobic |
| Provided Formats | Slant culture; Bacterial suspension; Plate culture |
| Culture Medium No. | 352239 |
| Culture Medium Name | Nutrient Agar |
| Culture Medium Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | 2-8°C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.