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BNCC

Bacillus spizizenii (Nakamura et al.) Dunlap et al.

SKU 293727

Bioware

A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.

Technical Specifications

OrganismBacillus spizizenii (Nakamura et al.) Dunlap et al.
Material TypeMicrobial Strain
Preservation FormFreeze dried pellet
Morphological CharacteristicsColony diameter: 0.5-1mm; circular with irregular margins; opaque; white upper surface; centrally raised; rough texture; moist consistency; easily lifted; G+ (blue-violet); bacillus; purity: pure
Culture Method① Prepare 1-2 fresh plates as described above; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it open; use forceps to crush it; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve completely, and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto the agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions; the strain is ready for use once colonies appear.
Culture Conditions30°C; 18-24h; aerobic
Provided FormatsAgar Slant; Bacterial suspension; Agar plate
Culture Medium No.352239
Culture Medium NameNutrient Agar
Culture Medium CompositionPeptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25°C)
ApplicationMicrobiological research, method verification, culture studies, and laboratory quality control.
Storage Conditions2-8°C
Country of OriginChina

Documents

The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.