BNCC
Ana-1 – Mouse macrophages
SKU 338182
Bioware
A characterized cultured-cell model intended for controlled in vitro research and analytical applications.
Suitable for cell-based assay development, method verification, comparative studies, and laboratory research.
Technical Specifications
| Organism | Ana-1 |
|---|---|
| Material Type | Cell Line |
| Preservation Form | frozen vial |
| Morphological Characteristics | Lymphoblast-like，Round，Small clusters，Cellular secretory black granules |
| Culture Method | Resuscitation steps: ① Remove the frozen vial from liquid nitrogen or -80 °C refrigerator and place it in PE gloves. Quickly immerse it in a 37 °C water bath, shake the vial to accelerate dissolution, and dissolve it completely within 1 minute; ② Add the dissolved cell solution into a centrifuge tube containing 9mL of complete medium on a ultra-clean bench, centrifuge at 1000-1200rpm for 3-5 minutes, discard the supernatant, and resuspend the cells in 1-2mL of complete medium. ③ Add the cell suspension into a T25 flask containing 6-7mL of complete medium and place the flask vertically in an incubator. Cell passage: ① Centrifuge method: Collect cells, centrifuge at 1000rpm for 5 minutes, discard the supernatant, add 1-2mL of culture medium and blow evenly. Divide the cell suspension into fresh T25 flasks containing 8mL of culture medium at a ratio of 1:2.② Half volume liquid exchange method: Please gently aspirate half of the supernatant culture medium, resuspend the remaining culture medium with cell sediment, and divide the cell suspension into fresh T25 flask containing 8mL of culture medium in a ratio of 1:2.③ Pay attention to changes in the pH value of the culture medium and cell density, change the medium regularly (2-3 times a week), and repeat the passage operation or cryopreservation when the cell density reaches greater than 2 × 10^6 cells/mL. |
| Culture Conditions | 37°C; 5% CO₂ + 95% air |
| Provided Formats | cryovial |
| Culture Medium No. | 338360 |
| Culture Medium Name | RPMI-1640 Complete Medium (with 10% FBS) |
| Culture Medium Composition | 90%RPMI-1640+10%FBS |
| Application | Cell culture research, method development, assay validation, and laboratory research. |
|---|---|
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.