BNCC
ε15
SKU 373642
Bioware
A preserved microbial culture intended for controlled microbiological studies and reference use.
Suitable for culture-method verification, identification workflows, media performance assessment, and laboratory quality control.
Technical Specifications
| Organism | ε15 |
|---|---|
| Material Type | Microbial Strain |
| Preservation Form | Frozen vial |
| Culture Method | ① Host bacterial culture: Inoculate the host bacteria into the above culture medium and culture under the above conditions until they grow. ② Host bacteria preparation: Pick the host bacteria from the Agar plate, dissolve them in 40-50mL nutrient broth culture medium, shake at 37 °C and 160-180rpm until OD600 is 0.1-0.4; ③ Open the freeze-drying tube: Open it in the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ④ Dissolve Freeze dried pellet: Take 0.5mL of nutrient broth and transfer it into a freeze-drying tube. After sufficient dissolution, transfer it into a triangular bottle of host bacterial solution; ⑤ Phage culture: Place the triangular flask at 37 °C and incubate overnight on a shaker at 160-180rpm. After 16-18 hours, centrifuge the phage culture at 4000g for 10 minutes and filter the lysate through a 0.2 μ m sterile filter. The filtrate can be stored at 4 °C |
| Culture Conditions | 37°C； 18-24h; aerobic |
| Provided Formats | Bacterial solution |
| Culture Medium No. | 352252 |
| Culture Medium Name | Brain Heart Infusion Agar |
| Culture Medium Composition | Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na2HPO4:2.5g, Agar:13.5g, pH:7.4±0.2(25°C) |
| Application | Microbiological research, method verification, culture studies, and laboratory quality control. |
|---|---|
| Storage Conditions | -80 °C |
| Country of Origin | China |
Documents
The Certificate of Analysis (COA) is issued for the applicable batch or lot and is available upon request.